Tuning Deterministic Lateral Displacement Devices Using Dielectrophoresis

Fig. S1 Layout of the D-DLD with peripheral vacuum pump and electronic connections. (a) The device is fabricated in PDMS and glass using replica molding. (b) Micrographs of the entrance channels and a side-wall. (c) A scale drawing of the separation device. A stream of particles injected into the device is continuously separated about the critical diameter, D c. Particles smaller than the critical diameter follow the direction of the fluid flow (red) whereas particles larger than the critical diameter follow the geometry of the post array (green). The black frame is a fluorescent micrograph (from movie m1.mov, ESI) showing actual separation of 3 µm (red) and 5 µm (green) diameter polystyrene beads. At this early stage the separated beads are extracted via a single exit for simplicity but for actual separation at least two exits will be used.